Combination therapy of intracisternal magnesium sulfate infusion and intravenous hydrogen-enriched fluid administration demonstrates safety in normal and subarachnoid hemorrhage model rats, with no adverse effects on physiological parameters including blood gases, intracranial pressure, blood pressure, and temperature. This finding supports the therapeutic potential of this combination approach for neuroprotection against delayed ischemic neurological deficits following subarachnoid hemorrhage without compromising normal physiological function.
Plain-Language Summary
Researchers tested a combination treatment in rats that involved injecting magnesium sulfate directly into the fluid surrounding the brain and giving hydrogen-enriched fluid intravenously (through the vein). They measured whether this treatment affected important body functions like blood oxygen levels, brain pressure, blood pressure, and temperature in both healthy rats and rats with a type of brain injury called subarachnoid hemorrhage. The treatment did not cause any harmful changes to these physiological measurements or visible damage to brain tissue.
Abstract
Combination therapy of intracisternal (ic) infusion of magnesium sulfate solution and intravenous (iv) injection of hydrogen-enriched fluid can provide protection against delayed ischemic neurological deficits after subarachnoid hemorrhage (SAH). However, the effects of this therapy on physiological parameters in vivo remain unclear. In this study, the effects of combination therapy were investigated on the physiological parameters of normal and experimental SAH model rats. SAH was induced by 0.1 mL autologous blood injection into the cisterna magna 24 hours prior to combination therapy. Male Sprague-Dawley rats received simultaneous ic infusion and iv injection as follows: control group, ic infusion of lactated Ringer solution and iv injection of glucose-electrolyte solution (SOLDEM1®); Mg group, ic infusion of magnesium sulfate solution and iv injection of SOLDEM1®; and Mg+H2 and SAH groups, ic infusion of magnesium sulfate solution and iv injection of hydrogen-enriched fluid. Blood gases, intracranial pressure, blood pressure, and rectal temperature were measured. The physiological values were all within normal ranges and no significant changes were found in all groups. No particular histological findings were observed. Combination therapy of ic infusion of magnesium and iv administration of hydrogen has no harmful effect on the physiological parameters of rats.
